mcp 1 elisa kit  (Elabscience Biotechnology)

Journal: Renal Failure

Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

doi: 10.1080/0886022X.2026.2653954

Figure Lengend Snippet: BAI increased the cell viability and inhibiting the apoptosis, inflammation, and oxidative stress in ZAS-induced podocyte injury. (A-B) The viability and apoptosis of MPC-5 cells were assessed using CCK-8 (A) and flow cytometry (B) after induction with 10% ZAS for 1 h followed by treatment with 2 µmol/L or 10 µmol/L BAI for 24 h. (C-E) The levels of pro-inflammatory cytokines TNF-α (C), IL-6 (D), and MCP-1 (E) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. (F) The levels of ROS in MPC-5 cells were evaluated by flow cytometry following treatment with ZAS and BAI. (G-H) The level of T-SOD (G) and MDA (H) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Renal Failure

Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

doi: 10.1080/0886022X.2026.2653954

Figure Lengend Snippet: BAI mitigated the inflammation of IMN mice. (A) After being treated with 20 mg/kg and 100 mg/kg BAI, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) After being treated with BAI, the level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) After being treated with BAI, the serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). * p < 0.05, ** p < 0. 01, *** p < 0.001.

Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

Techniques: Histopathology, Staining, Enzyme-linked Immunosorbent Assay

Journal: Renal Failure

Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

doi: 10.1080/0886022X.2026.2653954

Figure Lengend Snippet: BAI suppressed the inflammation and oxidative stress of ZAS-induced podocytes by inactivating the AGE/RAGE pathway. (A-B) After being 10% ZAS induced for 1 h and 2 µmol/L BAI treated for 24 h, the expression of AGE (A) and RAGE (B) in MPC-5 cells were quantified. (C) After being treated with 2 µmol/L BAI and 2 µg/mL AGE-BSA for 24 h, the expression of RAGE in ZAS-induced MPC-5 cells were quantified using western blot assay. (D) The viability of ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment was determined using CCK-8 assay. (E-G) The levels of pro-inflammatory cytokines TNF-α (E), IL-6 (F), and MCP-1 (G) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. (H-I) The level of T-SOD (H) and MDA (I) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

Techniques: Expressing, Western Blot, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

Journal: Renal Failure

Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

doi: 10.1080/0886022X.2026.2653954

Figure Lengend Snippet: BAI mitigated the inflammation and oxidative stress of IMN mice via AGE/RAGE pathway. (A) After being treated with 20 mg/kg BAI and 20 mg/kg AGE-BSA, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) The level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) The serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. (F) The LDH level in the kidney tissue of IMN mice was detected using a commercial reagent kit. (G-H) The levels of T-SOD (G) and MDA (H) in kidney tissue of IMN mice was detected by ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). ** p < 0.01, *** p < 0.001.

Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

Techniques: Histopathology, Staining, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Timing of coffee consumption and insulin resistance: evidence from human and animal studies

doi: 10.3389/fimmu.2026.1775412

Figure Lengend Snippet: Time-dependent effects of coffee administration on IR in mice. (A) Illustration of the experimental study design; (B) Serum insulin and fasting blood glucose (6-hour fast) levels; (C) Intraperitoneal glucose tolerance test (ipGTT) curves; (D) Pro-inflammatory cytokines (IL-1β, IL-6, ICAM-1, and MCP-1) levels. Data are mean ± SEM (n=8/group). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-hoc test * p < 0.05, **p<0.01, and *** p < 0.001.HFD, high-fat diet; IR, insulin resistance.

Article Snippet: Mouse IL-1β, IL-6, ICAM-1, MCP-1 ELISA kits (E-EL-M0037, E-HSEL-M0005, E-EL-M3037, E-EL-M3001, Elabscience) were used to detect the levels of inflammatory factors in mouse serum.

Techniques:

Journal: Redox Biology

Article Title: The electrophilic metabolite of kynurenine, kynurenine-CKA, requires C151 in Keap1 to derepress Nrf2

doi: 10.1016/j.redox.2026.104009

Figure Lengend Snippet: AhR inhibition has no effect on the anti-inflammatory actions of Kyn-CKA in BMDMs. (A) Mouse AhR reporter cells expressing luciferase under the control of the xenobiotic response element (XRE) were incubated with Kyn-CKA or kynurenine for 24 h prior to luminescence measurement (n = 3). Data were fitted to a sigmoidal four-parameter logistic curve (L-Kyn: r 2 = 0.824, IC 50 = 28 μM, span = 3218 RFU. Kyn-CKA: r 2 = 0.970, IC 50 = 13 μM, span = 47,901 RFU). (B) Inhibition of AhR-dependent luciferase expression by CH223191 (AhRinh). (C–F) Kyn-CKA inhibits the expression of the NF-κB-regulated genes IL6, MCP1, Nos2 and IL1β in BMDM following 5 h incubation with LPS (0.5 μg/mL) and the indicated concentrations of Kyn-CKA both in the presence or absence of CH223191 (10 μM). Data are n = 3–4, ∗∗∗∗p < 0.0001 by one-way ANOVA and Tukey's post-test. (G – H) Extracellular cytokine levels from BMDM treated as in C–F for 5 h. Data are n = 3–4 (all replicates shown), ∗∗∗∗p < 0.0001 by one-way ANOVA and Tukey's post-test.

Article Snippet: ELISA kits for MCP1 (DY479 and MJE00B) and IL6 (DY406) were obtained from R&D Systems (Minneapolis, MN).

Techniques: Inhibition, Expressing, Luciferase, Control, Incubation

Journal: Redox Biology

Article Title: The electrophilic metabolite of kynurenine, kynurenine-CKA, requires C151 in Keap1 to derepress Nrf2

doi: 10.1016/j.redox.2026.104009

Figure Lengend Snippet: The low-dose anti-inflammatory effects of Kyn-CKA in BMDMs are dependent on Nrf2. (A – B) Treatment with Kyn-CKA (5 h) fails to induce the expression of Nqo1 and Gclm in BMDMs obtained from Nrf2-knockout (Nrf2 −/− ) mice. Data are n = 3, ∗∗∗∗p < 0.0001 by two-way ANOVA and Tukey's post-test. (C – G) Effects of Kyn-CKA on the expression of NF-κB−regulated genes in WT and Nrf2-KO BMDMs following 5 h incubation with LPS (0.5 μg/mL) and the indicated concentrations of Kyn-CKA. Data are n = 6, ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 by two-way ANOVA and Tukey's post-test. Nrf2 was identified as a significant source of variation for MCP1, IL6, TNFα, Nos2, (p < 0.0001) and IL1β (p < 0.005).

Article Snippet: ELISA kits for MCP1 (DY479 and MJE00B) and IL6 (DY406) were obtained from R&D Systems (Minneapolis, MN).

Techniques: Expressing, Knock-Out, Incubation